School of Mathematics and Statistics

Postgraduate research

Research being undertaken by our current postgraduates:

Contact

Ibrahim Almanjahie


Start date

Mar 2009

Submission date

Ibrahim Almanjahie

Thesis

Statistical Modelling and Analysis of Ion Channel Data

Summary

Ion channels are specialised protein molecules which selectively control the movement of ions and molecules across biological membranes, thereby regulating many aspects of cell function (Aidley et al., 1996).

Understanding the behaviour of ion channels will provide insight into such regulation and its pathologies, for example as a result of genetic or other disease, and into possible interventions, for example through drugs, which may modify channel behaviour and consequently cell functioning (Hille, 1992). Passage of the ions and molecules occurs through aqueous pores gated usually in an all-or-none fashion by specific stimuli, including mechanical stress. One type of channel is the mechanosensitive large conductance channel (MscL) in the bacterium E. Coli. The refined patch clamp technique (Hamill et al., 1981) allows channel ionic currents to be recorded. Together with this, determination of the structure of MscL and developments in cloning capability %(Sukharev et al., 1994) have enabled unprecedented study of MscL functional behaviour. In turn, the extensive high quality patch clamp data allows model building and testing of basic mechanisms of channel kinetics.

Limited research has been done in analysing Patch clamp data from the large conductance mechanosensitive channel (MscL) in E. Coli due to a number of challenges (e.g. suitable bandwidth, multi-level channel data, correlated noise). This thesis will focus on using higher bandwidth Patch clamp data from MscL with the aim of developing a strategy for statistical analysis based on hidden Markov models (HMMs), incorporating correlated noise, and determining the number of conductance levels of channel, together with mean current, mean dwell time and equilibrium probability of occupancy for each level.

Why my research is important

Understanding the behaviour of ion channels will provide insight into such regulation and its pathologies, for example as a result of genetic or other disease, and into possible interventions, for example through drugs, which may modify channel behaviour and consequently cell functioning.



Statistics clinic

Assistance in statistics is available for Postgraduates students by research at the UWA Centre for Applied Statistics.

 

School of Mathematics and Statistics

This Page

Last updated:
Monday, 16 June, 2014 2:41 PM

http://www.maths.uwa.edu.au/275877